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OsLSD1, a Rice Zinc Finger Protein, Regulates Programmed Cell Death and Callus Differentiation

May 2005 , Volume 18 , Number  5
Pages  375 - 384

Lijuan Wang , 1 , 2 Zhongyou Pei , 1 Yingchuan Tian , 1 and Chaozu He 1 , 3

1National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, P. R. China; 2Graduate School of the Chinese Academy of Sciences, Beijing 100039, P. R. China; 3Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, P. R. China

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Accepted 30 November 2004.

The Arabidopsis LSD1 and LOL1 proteins both contain three conserved zinc finger domains and have antagonistic effects on plant programmed cell death (PCD). In this study, a rice (Oryza sativa) functional homolog of LSD1, designated OsLSD1, was identified. The expression of OsLSD1 was light-induced or dark-suppressed. Overexpression of OsLSD1 driven by the cauliflower mosaic virus 35S promoter accelerated callus differentiation in transformed rice tissues and increased chlorophyll b content in transgenic rice plants. Antisense transgenic rice plants exhibited lesion mimic phenotype, increased expression of PR-1mRNA, and an accelerated hypersensitive response when inoculated with avirulent isolates of blast fungus. Both sense and antisense transgenic rice plants conferred significantly enhanced resistance against a virulent isolate of blast fungus. Moreover, ectopic overexpression of OsLSD1 in transgenic tobacco (Nicotiana tabacum) enhanced the tolerance to fumonisins B1 (FB1), a PCD-eliciting toxin. OsLSD1 green fluorescent protein fusion protein was located in the nucleus of tobacco cells. Our results suggest that OsLSD1 plays a negative role in regulating plant PCD, whereas it plays a positive role in callus differentiation.

© 2005 The American Phytopathological Society