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Glutathione and Homoglutathione Play a Critical Role in the Nodulation Process of Medicago truncatula

March 2005 , Volume 18 , Number  3
Pages  254 - 259

Pierre Frendo , Judith Harrison , Christel Norman , María Jesús Hernández Jiménez , Ghislaine Van de Sype , Alain Gilabert , and Alain Puppo

Interactions Plantes-Microorganismes et Santé Végétale, UMR CNRS-INRA-Université de Nice-Sophia Antipolis, 400, Route des Chappes, BP167, 06903 Sophia-Antipolis Cedex, France

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Accepted 1 November 2004.

Legumes form a symbiotic interaction with bacteria of the Rhizobiaceae family toproduce nitrogen-fixing root nodules under nitrogen-limiting conditions. This process involves the recognition of the bacterial Nod factors by the plant which mediates the entry of the bacteria into the root and nodule organogenesis. We have examined the importance of the low molecular weight thiols, glutathione (GSH) and homoglutathione (hGSH), during the nodulation process in the model legume Medicago truncatula. Using both buthionine sulfoximine, a specific inhibitor of GSH and hGSH synthesis, and transgenic roots expressing GSH synthetase and hGSH synthetase in an antisense orientation, we showed that deficiency in GSH and hGSH synthesis inhibited the formation of the root nodules. This inhibition was not correlated to a modification in the number of infection events or to a change in the expression of the Rhizobium sp.-induced peroxidase rip1, indicating that the low level of GSH or hGSH did not alter the first steps of the infection process. In contrast, a strong diminution in the number of nascent nodules and in the expression of the early nodulin genes, Mtenod12 and Mtenod40, were observed in GSHand hGSH-depleted plants. In conclusion, GSH and hGSH appear to be essential for proper development of the root nodules during the symbiotic interaction.

© 2005 The American Phytopathological Society