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Selection of Heterodera glycines Chorismate Mutase-1 Alleles on Nematode-Resistant Soybean

June 2005 , Volume 18 , Number  6
Pages  593 - 601

Kris N. Lambert , 1 Sadia Bekal , 1 Leslie L. Domier , 1 , 2 Terry L. Niblack , 1 Gregory R. Noel , 1 , 2 and Charles A. Smyth 1

1Department of Crop Science and 2United States Department of Agriculture-Agricultural Research Service, University of Illinois at Urbana-Champaign, 1102 South Goodwin Avenue, Urbana 61801, U.S.A.

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Accepted 20 January 2005.

The soybean cyst nematode Heterodera glycines is the most destructive pathogen of soybean in the Unites States. Diversity in the parasitic ability of the nematode allows it to reproduce on nematode-resistant soybean. H. glycines chorismate mutase-1 (Hg-CM-1) is a nematode enzyme with the potential to suppress host plant defense compounds; therefore, it has the potential to enhance the parasitic ability of nematodes expressing the gene. Hg-cm-1 is a member of a gene family where two alleles, Hg-cm-1A and Hg-cm-1B, have been identified. Analysis of the Hg-cm-1 gene copy number revealed that there are multiple copies of Hg-cm-1 alleles in the H. glycines genome. H. glycines inbred lines were crossed to ultimately generate three F2 populations of second-stage juveniles (J2s) segregating for Hg-cm-1A and Hg-cm-1B. Segregation of Hg-cm-1A and 1B approximated a 1:2:1 ratio, which suggested that Hg-cm-1 is organized in a cluster of genes that segregate roughly as a single locus. The F2H. glycines J2 populations were used to infect nematode- resistant (Hartwig, PI88788, and PI90763) and susceptible (Lee 74) soybean plants. H. glycines grown on Hartwig, Lee 74, and PI90763 showed allelic frequencies similar to Hg-cm-1A/B, but nematodes grown on PI88788 contained predominately Hg-cm-1A allele as a result of a statistically significant drop of Hg-cm-1B in the population. This result suggests that specific Hg-cm-1 alleles, or a closely linked gene, may aid H. glycines in adapting to particular soybean hosts.

Additional keywords: parasitism gene , resistance , shikimate pathway .

© 2005 The American Phytopathological Society