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Functional Analysis of Pathogenicity Proteins of the Potato Cyst Nematode Globodera rostochiensis Using RNAi

July 2005 , Volume 18 , Number  7
Pages  621 - 625

Qing Chen , 1 S. Rehman , 2 G. Smant , 2 and John T. Jones 1

1Plant-Pathogen Interactions Programme, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, U.K.; 2Laboratory of Nematology, Department of Plant Sciences, Wageningen University, Binnenhaven 5, 6709PD Wageningen, The Netherlands

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Accepted 15 February 2005.

RNA interference (RNAi) has been used widely as a tool for examining gene function and a method that allows its use with plant-parasitic nematodes recently has been described. Here, we use a modified method to analyze the function of secreted β-1,4, endoglucanases of the potato cyst nematode Globodera rostochiensis, the first in vivo functional analysis of a pathogenicity protein of a plant-parasitic nematode. Knockout of the β-1,4, endoglucanases reduced the ability of the nematodes to invade roots. We also use RNAi to show that gr-ams-1, a secreted protein of the main sense organs (the amphids), is essential for host location.

Additional keywords: amphid-secreted protein , cellulase , gene silencing , invasion .

© 2005 The American Phytopathological Society