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GmCOI1, a Soybean F-Box Protein Gene, Shows Ability to Mediate Jasmonate-Regulated Plant Defense and Fertility in Arabidopsis

December 2005 , Volume 18 , Number  12
Pages  1,285 - 1,295

Zhilong Wang , 1 , 2 Liangying Dai , 1 , 3 Zide Jiang , 1 , 4 Wen Peng , 1 Lianhui Zhang , 1 Guoliang Wang , 5 , 6 and Daoxin Xie 1 , 2

1Institute of Molecular and Cell Biology, 61 Biopolis drive, 138673 Singapore; 2Department of Biological Sciences, National University of Singapore, 117543 Singapore; 3College of Bio-Safety Science and Technology, Hunan Agricultural University, 410128 China; 4Department of Plant Pathology, South China Agricultural University, 510642 China; 5Rice Genomics Laboratory, Hunan Agricultural University, 410128 China; 6Department of Plant Pathology, The Ohio State University, Columbus, OH 43210, U.S.A.


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Accepted 15 August 2005.

The F-box protein gene COI1 from Arabidopsis plays a fundamental role in response to jasmonates, which regulate plant root growth, pollen fertility, wounding and healing, and defense against pathogens and insects. Null mutations in COI1 were previously found to abolish all the jasmonate responses, and the Arabidopsis coi1-1 mutant is male sterile and susceptible to pathogen infection. In this study, we isolated an F-box protein gene from soybean, which shares significant homology with the Arabidopsis COI1 and similarly contains an F-box motif and leucine rich repeats (LRR), here designated GmCOI1 (Glycine max L. (Merr.) COI1). To test whether the sequence homology and structural similarity are indicative of functional conservation, we expressed GmCOI1 in the Arabidopsis coi1-1 mutant. The transgenic coi1-1 plants with expression of the GmCOI1 gene were found to exhibit normal jasmonate responses, including jasmonate-regulated plant defense and fertility. In addition, the chimerical proteins with swapped domain of the F-box motif or LRR between GmCOI1 and COI1 were shown to functionally complement the coi1-1 mutation. Furthermore, GmCOI1 was found to assemble into the Skp1-Cullin-F-box (SCF) complexes, similar to the formation of the Arabidopsis SCFCOI1. These data demonstrate the soybean F-box protein gene GmCOI1 is able to mediate jasmonate-regulated plant defense and fertility in Arabidopsis, which implies a generic jasmonate pathway with conserved signal components in different plant species.



© 2005 The American Phytopathological Society