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Stimulation of the Lipoxygenase Pathway Is Associated with Systemic Resistance Induced in Bean by a Nonpathogenic Pseudomonas Strain

September 2004 , Volume 17 , Number  9
Pages  1,009 - 1,018

Marc Ongena , 1 Francéline Duby , 2 Fanny Rossignol , 1 Marie-Laure Fauconnier , 3 Jacques Dommes , 2 and Philippe Thonart 1

1Centre Wallon de Biologie Industrielle, Université de Liège and Faculté Universitaire des Sciences Agronomiques de Gembloux, Belgium; 2Laboratoire de Biologie Moléculaire Végétale, Université de Liège, Belgium; 3Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, Belgium

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Accepted 7 May 2004.

Systemic defense reactions induced in bean by the nonpathogenic Pseudomonas putida BTP1 strain reduced disease caused by Botrytis cinerea. Phenylalanine ammonialyase activity and the level of endogenous free salicylic acid were compared in plant growth-promoting rhizobacteria-treated versus control plants, but no significant differences were detected. Furthermore, no enhanced fungitoxicity was detected in methanolic leaf extracts, suggesting that accumulation of bean phytoalexins was not part of the stimulated defense mechanisms. However, BTP1-inoculated plants showed increased levels of both linoleic and linolenic acids. On this basis, we further investigated whether the lipoxygenase pathway, leading to antifungal phytooxylipins, could have been stimulated. Two key enzymatic activities of this metabolic route, namely lipoxygenase and hydroperoxide lyase, were significantly stimulated during the first four days after challenging BTP1-treated plants with the pathogen. This was observed in parallel with a more rapid consumption of the respective substrates of these enzymes, as revealed by measurements of endogenous concentrations of linolenic acid and their hydroperoxide derivatives. Moreover, headspace-gas chromatography analyses showed significantly higher concentrations of the fungitoxic final product Z-3-hexenal in leaves from BTP1-inoculated beans as compared with control plants. Taken together, these results strongly suggest that the oxylipin pathway can be associated with enhanced disease resistance induced in bean plants by nonpathogenic rhizobacteria.

Additional keyword: plant protection.

© 2004 The American Phytopathological Society