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Candidate Defense Genes as Predictors of Quantitative Blast Resistance in Rice

October 2004 , Volume 17 , Number  10
Pages  1,146 - 1,152

Bin Liu , 1 , 3 , 4 Shaohong Zhang , 1 Xiaoyuan Zhu , 2 Qiyun Yang , 2 Shangzhong Wu , 2 Mantong Mei , 3 Ramil Mauleon , 4 Jan Leach , 5 Tom Mew , 4 and Hei Leung 4

1Rice Research Institute and 2Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P. R. China; 3College of Life Sciences, South China Agricultural University, Guangzhou 510642, P. R. China; 4Entomology and Plant Pathology Division, International Rice Research Institute, DAPO Box 7777, Manila, Philippines; 5Department of Plant Pathology, Kansas State University, Manhattan 66506-5502, U.S.A.

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Accepted 25 June 2004.

Although quantitative trait loci (QTL) underpin many desirable agronomic traits, their incorporation into crop plants through marker-assisted selection is limited by the low predictive value of markers on phenotypic performance. Here we used candidate defense response (DR) genes to dissect quantitative resistance in rice using recombinant inbred (RI) and advanced backcross (BC) populations derived from a blast-resistant cultivar, Sanhuangzhan 2 (SHZ-2). Based on DNA profiles of DR genes, RI lines were clustered into two groups corresponding to level of resistance. Five DR genes, encoding putative oxalate oxidase, dehydrin, PR-1, chitinase, and 14-3-3 protein, accounted for 30.0, 23.0, 15.8, 6.7, and 5.5% of diseased leaf area (DLA) variation, respectively. Together, they accounted for 60.3% of the DLA variation and co-localized with resistance QTL identified by interval mapping. Average phenotypic contributions of oxalate oxidase, dehydrin, PR-1, chitinase, and 14-3-3 protein in BC lines were 26.1, 19.0, 18.0, 11.5, and 10.6%, respectively, across environments. Advanced BC lines with four to five effective DR genes showed enhanced resistance under high disease pressure in field tests. Our results demonstrate that the use of natural variation in a few candidate genes can solve a long-standing problem in rice production and has the potential to address other problems involving complex traits.

Additional keywords: defense response gene, Magnaporthe grisea.

© 2004 The American Phytopathological Society