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Tobacco Genes Induced by the Bacterial Effector Protein AvrPto

October 2004 , Volume 17 , Number  10
Pages  1,139 - 1,145

Venkatappa K. Thara , 1 Alexander R. Seilaniantz , 1 Youping Deng , 2 Yinghua Dong , 2 Yinong Yang , 3 Xiaoyan Tang , 1 and Jian-Min Zhou 1

1Department of Plant Pathology, Kansas State University, Manhattan, KS, U.S.A.; 2Division of Biology, Kansas State University, Manhattan, KS, U.S.A.; 3Department of Plant Pathology, University of Arkansas, Fayetteville, AR, U.S.A.

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Accepted 28 June 2004.

The type III effector protein AvrPto acts as a virulence factor in susceptible plants lacking a cognate resistance gene but triggers hypersensitive response and disease resistance in tomato plants carrying the Pto gene or in tobacco plants carrying an unknown resistance gene. To assist the characterization of cellular responses caused by AvrPto in the plant, a pathogen-free system was adopted to isolate genes up-regulated 12 h after induced expression of AvrPto. By using subtraction cloning and transgenic tobacco plants expressing avrPto as a transgene, we isolated 125 nonredundant cDNA clones that represent avrPto-response genes (ARG). In addition to genes that are known to be induced by Pto-avrPto recognition, a number of new genes were also isolated. Most of ARG showed a specific induction in tobacco plants challenged with incompatible or nonhost pathogens. The use of an avrPto mutant that selectively eliminated the avrPto recognition in tobacco demonstrated that the ARG were induced in a highly specific manner by the avirulence, instead of the virulence activity of avrPto.

Additional keywords: subtracted cDNA library, Pseudomonas syringae.

© 2004 The American Phytopathological Society