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Cloning, Characterization, and Evolution of the NBS-LRR-Encoding Resistance Gene Analogue Family in Polyploid Cotton (Gossypium hirsutum L.)

November 2004 , Volume 17 , Number  11
Pages  1,234 - 1,241

Limei He , 1 Chunguang Du , 2 Lina Covaleda , 1 Zhanyou Xu , 1 A. Forest Robinson , 3 John Z. Yu , 3 Russell J. Kohel , 3 and Hong-Bin Zhang 1

1Department of Soil and Crop Sciences and Institute for Plant Genomics and Biotechnology, 2123 TAMU, Texas A&M University, College Station, U.S.A.; 2Department of Biology and Molecular Biology, Montclair State University, Upper Montclair, NJ, U.S.A.; and 3USDA-ARS, SPARC, Cotton Pathology and Crop Germplasm Research Units, 2765 F&B Road, College Station, TX, U.S.A.

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Accepted 29 July 2004.

The nucleotide-binding site-leucine-rich repeat (NBS-LRR)-encoding gene family has attracted much research interest because approximately 75% of the plant disease resistance genes that have been cloned to date are from this gene family. We cloned the NBS-LRR-encoding genes from polyploid cotton by a polymerase chain reaction-based approach. A sample of 150 clones was selected from the NBS-LRR gene sequence library and was sequenced, and 61 resistance gene analogs (RGA) were identified. Sequence analysis revealed that RGA are abundant and highly diverged in the cotton genome and could be categorized into 10 distinct subfamilies based on the similarities of their nucleotide sequences. The numbers of members vary many fold among different subfamilies, and gene index analysis showed that each of the subfamilies is at a different stage of RGA family evolution. Genetic mapping of a selection of RGA indicates that the RGA reside on a limited number of the cotton chromosomes, with those from a single subfamily tending to cluster and two of the RGA loci being colocalized with the cotton bacterial blight resistance genes. The distribution of RGA between the two subgenomes A and D of cotton is uneven, with RGA being more abundant in the A subgenome than in the D subgenome. The data provide new insights into the organization and evolution of the NBS-LRR-encoding RGA family in polyploid plants.

© 2004 The American Phytopathological Society