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RCH1, a Locus in Arabidopsis That Confers Resistance to the Hemibiotrophic Fungal Pathogen Colletotrichum higginsianum

July 2004 , Volume 17 , Number  7
Pages  749 - 762

Yoshihiro Narusaka , 1 , 2 , 3 Mari Narusaka , 1 , 4 Pyoyun Park , 2 Yasuyuki Kubo , 5 Takashi Hirayama , 3 , 4 , 6 Motoaki Seki , 3 , 4 Tomonori Shiraishi , 7 Junko Ishida , 4 Maiko Nakashima , 4 Akiko Enju , 4 Tetsuya Sakurai , 4 Masakazu Satou , 4 Masatomo Kobayashi , 8 and Kazuo Shinozaki 3 , 4

1Department of Biology, Tokyo Gakugei University, 4-1-1 Nukuikita-machi, Koganei-shi, Tokyo, 184-8501 Japan; 2Kobe University Graduate School of Science and Technology, 1-1 Rokkodai-cho, Nada-ku, Kobe, 657-8501 Japan; 3Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba, 305-0074 Japan; 4Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center (GSC), RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045 Japan; 5Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Prefectural University, 1-5 Shimogamonakaragi-cho, Sakyo-ku, Kyoto, 606-8522 Japan; 6Laboratory of Environmental Molecular Biology, Graduate School of Integrated Science, Yokohama City University, 1-7-29 Suehiro, Tsurumi, Yokohama, 230-0045 Japan; 7Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, 1-1-1 Tsushimanaka, Okayama, 700-8530 Japan; 8Experimental Plant Division, RIKEN Bio Resource Center, 3-1-1 Koyadai, Tsukuba, 305-0074 Japan

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Accepted 20 February 2004.

When challenged with the crucifer pathogen Colletotrichum higginsianum, Arabidopsis thaliana ecotype Columbia (Col-0) was colonized by the fungus within 2 to 3 days, developing brown necrotic lesions surrounded by a yellow halo. Lesions spread from the inoculation site within 3 to 4 days, and subsequently continued to expand until they covered the entire leaf. Electron microscopy confirmed that C. higginsianum is a hemibiotroph on Arabidopsis, feeding initially on living cells as a biotroph before switching to a necrotrophic mode of growth. A collection of 37 ecotypes of Arabidopsis varied in their responses to infection by C. higginsianum. The ecotype Eil-0 was highly resistant, with symptoms limited to necrotic flecking and with only very limited fungal colonization. Analyses suggested that the hypersensitive response and reactive oxygen species may be important in this defense response. Expression analyses with cDNA microarrays indicated that the defense reaction depends primarily on the jasmonic acid- and ethylene-dependent signaling pathways and, to a lesser extent, on the salicylate-dependent pathway. Crosses between the Eil-0 and Col-0 ecotypes suggested that the resistance in Eil-0 was dominant and was conferred by a single locus, which we named RCH1. RCH1 is the first resistance locus to be identified from Arabidopsis against the hemibiotrophic fungus genus Colletotrichum.

© 2004 The American Phytopathological Society