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The Arabidopsis TIR-NB-LRR Gene RAC1 Confers Resistance to Albugo candida (White Rust) and Is Dependent on EDS1 but not PAD4

July 2004 , Volume 17 , Number  7
Pages  711 - 719

Mohammad H. Borhan , 1 Eric B. Holub , 2 Jim L. Beynon , 2 Kevin Rozwadowski , 1 and S. Roger Rimmer 1

1Agriculture and Agri-Food Canada, Saskatoon Research Center, Saskatoon, SK., S7N 0X2, Canada; 2Horticulture Research International, Wellesbourne, Warwickshire, CV35 9EF, U.K.

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Accepted 25 January 2004.

Resistance to Albugo candida isolate Acem1 is conferred by a dominant gene, RAC1, in accession Ksk-1 of Arabidopsis thaliana. This gene was isolated by positional cloning and is a member of the Drosophila toll and mammalian interleukin-1 receptor (TIR) nucleotide-binding site leucine-rich repeat (NB-LRR) class of plant resistance genes. Strong identity of the TIR and NB domains was observed between the predicted proteins encoded by the Ksk-1 allele and the allele from an Acem1-susceptible accession Columbia (Col) (99 and 98%, respectively). However, major differences between the two predicted proteins occur within the LRR domain and mainly are confined to the β-strand/β-turn structure of the LRR. Both proteins contain 14 imperfect repeats. RAC1-mediated resistance was analyzed further using mutations in defense regulation, including: pad4-1, eds1-1, and NahG, in the presence of the RAC1 allele from Ksk-1. White rust resistance was completely abolished by eds1-1 but was not affected by either pad4-1 or NahG.

The American Phytopathological Society, 2004