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Cytological, Genetic, and Molecular Analysis to Characterize Compatible and Incompatible Interactions Between Medicago truncatula and Colletotrichum trifolii

August 2004 , Volume 17 , Number  8
Pages  909 - 920

Carine Torregrosa , 1 Stéphanie Cluzet , 1 Joëlle Fournier , 1 Thierry Huguet , 2 Pascal Gamas , 2 Jean-Marie Prospéri , 3 Marie-Thérèse Esquerré-Tugayé , 1 Bernard Dumas , 1 and Christophe Jacquet 1

1UMR 5546 CNRS-UPS, Pôle de Biotechnologie Végétale, 24 Chemin de Borde Rouge, BP17 Auzeville, 31326 Castanet-Tolosan, France; 2LIPM, INRA-CNRS, 24 Chemin de Borde Rouge, BP27 Auzeville, 31326 Castanet-Tolosan, France; 3Station de Génétique et d'Amélioration des Plantes, INRA, Domaine de Melgueil, 34130 Mauguio, France

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Accepted 16 March 2004.

In this study, a new pathosystem was established using the model plant Medicago truncatula and Colletotrichum trifolii, the causal agent of anthracnose on Medicago sativa. Screening of a few M. truncatula lines identified Jemalong and F83005.5 as resistant and susceptible to Colletotrichum trifolii race 1, respectively. Symptom analysis and cytological studies indicated that resistance of Jemalong was associated with a hypersensitive response of the plant. The two selected lines were crossed, and inoculations with C. trifolii were performed on the resulting F1 and F2 progenies. Examination of the disease phenotypes indicated that resistance was dominant and was probably due to a major resistance gene. Molecular components of the resistance were analyzed through macroarray experiments. Expression profiling of 126 expressed sequence tags corresponding to 92 genes, which were selected for their putative functions in plant defense or signal transduction, were compared in Jemalong and F83005.5 lines. A strong correlation was observed between the number of up-regulated genes and the resistance phenotype. Large differences appeared at 48 h postinoculation; more than 40% of the tested genes were up-regulated in the Jemalong line compared with only 10% in the susceptible line. Interestingly, some nodulin genes were also induced in the resistant line upon inoculation with C. trifolii.

Additional keywords: cell death, legume, phytoalexin, reactive oxygen species.

© 2004 The American Phytopathological Society