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A New Class of Ubiquitin Extension Proteins Secreted by the Dorsal Pharyngeal Gland in Plant Parasitic Cyst Nematodes

August 2004 , Volume 17 , Number  8
Pages  846 - 852

Tom Tytgat , 1 Bartel Vanholme , 2 Jan De Meutter , 3 Myriam Claeys , 1 Marjolein Couvreur , 1 Isabelle Vanhoutte , 3 Greetje Gheysen , 2 Wim Van Criekinge , 2 Gaetan Borgonie , 1 August Coomans , 1 and Godelieve Gheysen 2

1Department of Biology, Section Nematology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium; 2Department of Molecular Biotechnology, Faculty of Agricultural and Applied Biological Sciences, Ghent University, Coupure links 653, B-9000 Gent, Belgium; 3Flanders Interuniversity Institute for Biotechnology, Department of Plant Systems Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium


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Accepted 6 March 2004.

By performing cDNA AFLP on pre- and early parasitic juveniles, we identified genes encoding a novel type of ubiquitin extension proteins secreted by the dorsal pharyngeal gland in the cyst nematode Heterodera schachtii. The proteins consist of three domains, a signal peptide for secretion, a mono-ubiquitin domain, and a short C-terminal positively charged domain. A gfp-fusion of this protein is targeted to the nucleolus in tobacco BY-2 cells. We hypothesize that the C-terminal peptide might have a regulatory function during syncytium formation in plant roots.


Additional keyword: Globodera

© 2004 The American Phytopathological Society