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Genetic and Physical Localization of the Soybean Rpg1-b Disease Resistance Gene Reveals a Complex Locus Containing Several Tightly Linked Families of NBS-LRR Genes

September 2003 , Volume 16 , Number  9
Pages  817 - 826

Tom Ashfield , 1 Anna Bocian , 1 Dan Held , 1 Adam D. Henk , 1 Laura Fredrick Marek , 2 Dariush Danesh , 3 Silvia Peñuela , 3 Khalid Meksem , 4 David A. Lightfoot , 4 Nevin D. Young , 3 Randy C. Shoemaker , 2 , 5 and Roger W. Innes 1

1Department of Biology, Indiana University, Bloomington, IN 47405 U.S.A.; 2Department of Agronomy, Iowa State University, Ames IA 50010 U.S.A.; 3Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108 U.S.A.; 4Department of Plant, Soil and General Agriculture, Southern Illinois University, Carbondale, IL 62901 U.S.A.; 5USDA-ARS, Corn Insect and Crop Genetics Research Unit, Iowa State University, Ames IA 50010 U.S.A.

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Accepted 19 May 2003.

Alleles or tightly linked genes at the soybean (Glycine max L. Merr.) Rpg1 locus confer resistance to strains of Pseudomonas syringae pv. glycinea that express the avirulence genes avrB or avrRpm1. We have previously mapped Rpg1-b (the gene specific for avrB) to a cluster of resistance genes (R genes) with diverse specificities in molecular linkage group F. Here, we describe the high-resolution physical and genetic mapping of Rpg1-b to a 0.16-cM interval encompassed by two overlapping BAC clones spanning approximately 270 kilobases. Rpg1-b is part of a complex locus containing numerous genes related to previously characterized coiled coil-nucleotide binding site-leucine rich repeat (CC-NBS-LRR)-type R genes that are spread throughout this region. Phylogenetic and Southern blot analyses group these genes into four distinct subgroups, some of which are conserved in the common bean, Phaseolus vulgaris, indicating that this R gene cluster may predate the divergence of Phaseolus and Glycine. Members from different subgroups are physically intermixed and display a high level of polymorphism between soybean cultivars, suggesting that this region is rearranging at a high frequency. At least five CC-NBS-LRR-type genes cosegregate with Rpg1-b in our large mapping populations.

Additional keyword: RPMI.

© 2003 The American Phytopathological Society