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The Dual Role of the Potyvirus P3 Protein of Turnip mosaic virus as a Symptom and Avirulence Determinant in Brassicas

September 2003 , Volume 16 , Number  9
Pages  777 - 784

Carol E. Jenner , 1 Xiaowu Wang , 2 Kenta Tomimura , 3 Kazusato Ohshima , 3 Fernando Ponz , 2 and John A. Walsh 1

1Horticulture Research International, Wellesbourne, Warwick, CV35 9EF, U.K.; 2Departamento de Biotecnología, INIA, Autopista A-6 km7, 28040 Madrid, Spain; 3Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Saga 840-8502, Japan

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Accepted 28 May 2003.

Two isolates of the potyvirus Turnip mosaic virus (TuMV), UK 1 and CDN 1, differ both in their general symptoms on the susceptible propagation host Brassica juncea and in their ability to infect B. napus lines possessing a variety of dominant resistance genes. The isolate CDN 1 produces a more extreme mosaic in infected brassica leaves than UK 1 and is able to overcome the resistance genes TuRB01, TuRB04, and TuRB05. The resistance gene TuRB03, in the B. napus line 22S, is effective against CDN 1 but not UK 1. The nucleic acid sequences of the UK 1 and CDN 1 isolates were 90% identical. The C-terminal half of the P3 protein was identified as being responsible for the differences in symptoms in B. juncea. A single amino acid in the P3 protein was found to be the avirulence determinant for TuRB03. Previous work already has identified the P3 as an avirulence determinant for TuRB04. Our results increase the understanding of the basis of plant-virus recognition, show the importance of the potyviral P3 gene as a symptom determinant, and provide a role in planta for the poorly understood P3 protein in a normal infection cycle.

© 2003 The American Phytopathological Society