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Isolation of a Polygalacturonase-Inhibiting Protein (PGIP) from Wheat

November 2003 , Volume 16 , Number  11
Pages  955 - 961

Gabré Kemp , 1 Carl W. Bergmann , 2 Ron Clay , 3 Amie J. Van der Westhuizen , 1 and Zacharias A. Pretorius 1

1Department of Plant Sciences, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa; 2Complex Carbohydrate Research Center, 220 Riverbend Road, Athens, GA 30602-4712, U.S.A.; 3Department of Natural Sciences, Gainesville College, Gainesville, GA 30503, U.S.A.


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Accepted 2 July 2003.

Evidence for the presence of a polygalacturonase-inhibiting protein (PGIP) from a monocotyledonous cereal is presented. A 40.3-kDa PGIP that was closely associated with the cell wall was acetone-extracted and purified from wheat (Triticum aestivum L.) leaves and stems. Wheat PGIP exhibited a highly selective inhibitory activity against endopolygalacturonase (EPG) from various fungi. Of nine EPG tested, wheat PGIP only inhibited EPG from Cochliobolus sativus, a pathogen of the tribe Poaceae. A short N-terminal amino acid sequence of wheat PGIP shows no similarity to any other characterized PGIP.



© 2003 The American Phytopathological Society