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Two Potato Proteins, Including a Novel RING Finger Protein (HIP1), Interact with the Potyviral Multifunctional Protein HCpro

May 2003 , Volume 16 , Number  5
Pages  405 - 410

Deyin Guo , 1 , 4 Carl Spetz , 2 Mart Saarma , 1 and Jari P. T. Valkonen 2 , 3

1Institute of Biotechnology, PO Box 56, Viikki Biocenter, FIN-00014 University of Helsinki, Finland; 2Department of Plant Biology, Genetics Centre, SLU, SE-750 07 Uppsala, Sweden; 3Department of Applied Biology, PO Box 27, FIN-00014 University of Helsinki, Finland; 4College of Life Sciences, Modern Virology Center, Wuhan University, Wuhan 430072, China

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Accepted 11 December 2002.

Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions.

© 2003 The American Phytopathological Society