Jari P. T.
1Institute of Biotechnology, PO Box 56, Viikki Biocenter, FIN-00014 University of Helsinki, Finland; 2Department of Plant Biology, Genetics Centre, SLU, SE-750 07 Uppsala, Sweden; 3Department of Applied Biology, PO Box 27, FIN-00014 University of Helsinki, Finland; 4College of Life Sciences, Modern Virology Center, Wuhan University, Wuhan 430072, China
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Accepted 11 December 2002.
Potyviral helper-component proteinase (HCpro) is a multifunctional protein exerting its cellular functions in interaction with putative host proteins. In this study, cellular protein partners of the HCpro encoded by Potato virus A (PVA) (genus Potyvirus) were screened in a potato leaf cDNA library using a yeast two-hybrid system. Two cellular proteins were obtained that interact specifically with PVA HCpro in yeast and in the two in vitro binding assays used. Both proteins are encoded by single-copy genes in the potato genome. Analysis of the deduced amino acid sequences revealed that one (HIP1) of the two HCpro interactors is a novel RING finger protein. The sequence of the other protein (HIP2) showed no resemblance to the protein sequences available from databanks and has known biological functions.
© 2003 The American Phytopathological Society