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TIP, A Novel Host Factor Linking Callose Degradation with the Cell-to-Cell Movement of Potato virus X

February 2003 , Volume 16 , Number  2
Pages  132 - 140

Ingela Fridborg , 1 Jef Grainger , 1 Anthony Page , 1 Mark Coleman , 2 Kim Findlay , 3 and Susan Angell 1

1Department of Disease and Stress Biology, John Innes Centre, Norwich, NR4 7UH, U.K.; 2School of Biological Sciences, University of East Anglia, Norwich, U.K.; 3Department of Cell and Developmental Biology, John Innes Centre, Norwich, NR4 7UH, U.K.

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Accepted 29 October 2002.

The cell-to-cell movement of Potato virus X (PVX) requires four virus-encoded proteins, the triple gene block (TGB) proteins (TGB25K, TGB12K, and TGB8K) and the coat protein. TGB12K increases the plasmodesmal size exclusion limit (SEL) and may, therefore, interact directly with components of the cell wall or with plant proteins associated with bringing about this change. A yeast two-hybrid screen using TGB12K as bait identified three TGB12K-interacting proteins (TIP1, TIP2, and TIP3). All three TIPs interacted specifically with TGB12K but not with TGB25K or TGB8K. Similarly, all three TIPs interacted with β-1,3-glucanase, the enzyme that may regulate plasmodesmal SEL through callose degradation. Sequence analyses revealed that the TIPs encode very similar proteins and that TIP1 corresponds to the tobacco ankyrin repeat-containing protein HBP1. A TIP1::GFP fusion protein localized to the cytoplasm. Coexpression of this fusion protein with TGB12K induced cellular changes manifested as deposits of additional cytoplasm at the cell periphery. This work reports a direct link between a viral movement protein required to increase plasmodesmal SEL and a host factor that has been implicated as a key regulator of plasmodesmal SEL. We propose that the TIPs are susceptibility factors that modulate the plasmodesmal SEL.

Additional keywords: plasmodesmata, virus movement.

© 2003 The American Phytopathological Society