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Laminarin Elicits Defense Responses in Grapevine and Induces Protection Against Botrytis cinerea and Plasmopara viticola

December 2003 , Volume 16 , Number  12
Pages  1,118 - 1,128

Aziz Aziz , 1 Benoît Poinssot , 2 Xavier Daire , 2 Marielle Adrian , 2 , 3 Annie Bézier , 1 B. Lambert , 1 Jean-Marie Joubert , 4 and Alain Pugin 2

1Unité de Recherche Vignes et Vins de Champagne, URVVC - UPRES EA 2069, UFR Sciences, Moulin de la Housse, Université de Reims Champagne-Ardenne, BP 1039, F-51687 Reims cedex 2 France; 2Unité Mixte de Recherche, Plante-Microbe-Environnement, INRA 1088/CNRS 2625/ Université de Bourgogne, 17 rue Sully, BP 86510, 21065 Dijon cedex, France; 3Institut Jules Guyot, UMR 1088 INRA/Université de Bourgogne, 17 rue Sully, BP 86510, 21065 Dijon cedex, France; 4Société Goëmar, Avenue du Général Patton, BP 55, 35413 Saint Malo cedex, France

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Accepted 30 June 2003.

Grapevine (Vitis vinifera L.) is susceptible to many pathogens, such as Botrytis cinerea, Plasmopara viticola, Uncinula necator, and Eutypa lata. Phytochemicals are used intensively in vineyards to limit pathogen infections, but the appearance of pesticide-resistant pathogen strains and a desire to protect the environment require that alternative strategies be found. In the present study, the β-1,3-glucan laminarin derived from the brown algae Laminaria digitata was shown both to be an efficient elicitor of defense responses in grapevine cells and plants and to effectively reduce B. cinerea and P. viticola development on infected grapevine plants. Defense reactions elicited by laminarin in grapevine cells include calcium influx, alkalinization of the extracellular medium, an oxidative burst, activation of two mitogen-activated protein kinases, expression of 10 defense-related genes with different kinetics and intensities, increases in chitinase and β-1,3-glucanase activities, and the production of two phytoalexins (resveratrol and ε-viniferin). Several of these effects were checked and confirmed in whole plants. Laminarin did not induce cell death. When applied to grapevine plants, laminarin reduced infection by B. cinerea and P. viticola by approximately 55 and 75%, respectively. Our data describing a large set of defense reactions in grapevine indicate that the activation of defense responses using elicitors could be a valuable strategy to protect plants against pathogens.

© 2003 The American Phytopathological Society