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Synergistic Effect of Deoxyanthocyanins from Symbiotic Fern Azolla spp. on hrmA Gene Induction in the Cyanobacterium Nostoc punctiforme

September 2002 , Volume 15 , Number  9
Pages  875 - 882

Michael F. Cohen , 1 Yasuko Sakihama , 1 Yojiro C. Takagi , 1 Toshio Ichiba , 2 and Hideo Yamasaki 1

1Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus, Nishihara, Okinawa 903-0213, Japan; 2Research & Development Division, Okinawa Industrial Technology Center (OITC), 12-2 Suzaki, Gushikawa, Okinawa 904-2234 Japan

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Accepted 19 April 2002.

The hrmA gene of the N2-fixing cyanobacterium Nostoc punctiforme functions in repressing the formation of transitory motile filaments, termed hormogonia, by plant-associated vegetative filaments. Here, we report that anthocyanins can contribute to induction of hrmA expression. Aqueous extract from fronds of the fern Azolla pinnata, a host of symbiotic Nostoc spp., was found to be a potent inducer of hrmA-luxAB in N. punctiforme strain UCD 328. The hrmA-luxAB inducing activities of A. pinnata, as well as Azolla filiculoides, were positively correlated with levels of frond deoxyanthocyanins. Analyses of the deoxyanthocyanins in frond extracts revealed, in order of predominance, an acetylated glycoside derivative of luteolinidin (m/z 475) and of apigeninidin (m/z 459) and minor amounts of a second luteolinidin derivative. At up to 150 μM, a purified preparation of deoxyanthocyanins only weakly induced hrmA-luxAB on its own, but mixtures with hrmA-luxAB inducers (A. filiculoides extract or the flavonoid naringin) synergistically doubled to tripled their inducing activities. These results suggest that appropriately localized deoxyanthocyanins could function in plant-mediated mechanisms for repressing Nostoc spp. hormogonium formation.

Additional keywords: symbiosis , Anthoceros , cycad , Gunnera , heterocyst , rhizobia .

© 2002 The American Phytopathological Society