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Ectopic Expression of Tsi1 in Transgenic Hot Pepper Plants Enhances Host Resistance to Viral, Bacterial, and Oomycete Pathogens

October 2002 , Volume 15 , Number  10
Pages  983 - 989

Ryoung Shin , Jeong Mee Park , Jong-Min An , and Kyung-Hee Paek

Graduate School of Biotechnology, Korea University, 1-5 ga Anam-dong, Sungbuk-gu, Seoul 136-701, Korea

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Accepted 10 June 2002.

In many plants, including hot pepper plants, productivity is greatly affected by pathogen attack. We reported previously that tobacco stress-induced gene 1 (Tsi1) may play an important role in regulating stress responsive genes and pathogenesis-related (PR) genes. In this study, we demonstrated that overexpression of Tsi1 gene in transgenic hot pepper plants induced constitutive expression of several PR genes in the absence of stress or pathogen treatment. The transgenic hot pepper plants expressing Tsi1 exhibited resistance to Pepper mild mottle virus (PMMV) and Cucumber mosaic virus (CMV). Furthermore, these transgenic plants showed increased resistance to a bacterial pathogen, Xanthomonas campestris pv. vesicatoria and also an oomycete pathogen, Phytophthora capsici. These results suggested that ectopic expression of Tsi1 in transgenic hot pepper plants enhanced the resistance of the plants to various pathogens, including viruses, bacteria, and oomycete. These results suggest that using transcriptional regulatory protein genes may contribute to developing broad-spectrum resistance in crop plants.

Additional keywords: pathogen resistance , transcription factor .

© 2002 The American Phytopathological Society