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A Mitogen-Activated Protein Kinase Gene (MGV1) in Fusarium graminearum Is Required for Female Fertility, Heterokaryon Formation, and Plant Infection

November 2002 , Volume 15 , Number  11
Pages  1,119 - 1,127

Zhanming Hou , 1 Chaoyang Xue , 1 Youliang Peng , 1 Talma Katan , 2 H. Corby Kistler , 3 and Jin-Rong Xu 1

1Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907, U.S.A.; 2Department of Plant Pathology, The Volcani Center, Bet Dagan 50250, Israel; 3USDA-ARS, Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108, U.S.A.

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Accepted 20 July 2002.

Fusarium graminearum is an important pathogen of small grains and maize in many areas of the world. Infected grains are often contaminated with mycotoxins harmful to humans and animals. During the past decade, F. graminearum has caused several severe epidemics of head scab in wheat and barley. In order to understand molecular mechanisms regulating fungal development and pathogenicity in this pathogen, we isolated and characterized a MAP kinase gene, MGV1, which is highly homologous to the MPS1 gene in Magnaporthe grisea. The MGV1 gene was dispensable for conidiation in F. graminearum but essential for female fertility during sexual reproduction. Vegetative growth of mgv1 deletion mutants was normal in liquid media but reduced on solid media. Mycelia of the mgv1 mutants had weak cell walls and were hypersensitive to cell wall degrading enzymes. Interestingly, the mgv1 mutants were self-incompatible when tested for heterokaryon formation, and their virulence was substantially reduced. The ability of the mutants to accumulate trichothecene mycotoxins on inoculated wheat was also greatly reduced. Our data suggest that MGV1 in F. graminearum is involved in multiple developmental processes related to sexual reproduction, plant infection, and cell wall integrity.

Additional keywords: fungal pathogenicity , Gibberella zeae

© 2002 The American Phytopathological Society