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PPI1: A Novel Pathogen-Induced Basic Region-Leucine Zipper (bZIP) Transcription Factor from Pepper

June 2002 , Volume 15 , Number  6
Pages  540 - 548

Sang Jik Lee , 1 Mi Yeon Lee , 1 So Young Yi , 2 Sang Keun Oh , 2 Soon Ho Choi , 1 Nam Han Her , 1 Doil Choi , 2 Byung Whan Min , 1 Seung Gyun Yang , 1 and Chee Hark Harn 1

1Biotechnology Center, Nong Woo Bio Co., Ltd., 537-17 Jeongdan, Ganam, Yeoju, Kyonggi 469-885, Korea; 2Genome Research Center, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-600, Korea

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Accepted 11 February 2002.

We have isolated a full-length cDNA, PPI1 (pepper-PMMV interaction 1), encoding a novel basic region-leucine zipper (bZIP) DNA-binding protein, from expressed sequence tags differentially expressed in Capsicum chinense PI257284 infected with Pepper mild mottle virus (PMMV). PPI1 encodes a predicted protein of 170 amino acids and contains a putative DNA-binding domain that shares significant amino acid identity with ACGT-binding domains of members of the bZIP DNA-binding protein family. PPI1 was localized in the nucleus and had transcriptional activation activity in yeast. Transcripts of the PPI1 gene were preferentially induced during an incompatible interaction by inoculation with PMMV, Pseudomonas syringae pv. syringae 61, and Xanthomonas campestris pv. vesicatoria race 3. However, the PPI1 gene was not induced by abiotic stressors that activate the plant defense-signaling pathway. Our data provide the first evidence that a bZIP transcription factor is preferentially induced by pathogen attack, suggesting that PPI1 may play a specific functional role in the regulation of expression of plant defense-related genes.

Additional keywords: suppression subtractive hybridization.

© 2002 The American Phytopathological Society