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Potato Gene Y-1 is an N Gene Homolog That Confers Cell Death Upon Infection with potato virus Y

July 2002 , Volume 15 , Number  7
Pages  717 - 727

Sabina Vidal , 1 Héctor Cabrera , 1 Robert A. Andersson , 1 Anna Fredriksson , 1 and Jari P. T. Valkonen 1 , 2

1Department of Plant Biology, Swedish University of Agricultural Sciences, PO Box 7080, SE-750 07 Uppsala, Sweden; and 2Department of Applied Biology, University of Helsinki, Finland

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Accepted 19 March 2002.

ADG2 is a DNA sequence mapped to a resistance (R) generich region at the distal end of chromosome XI in potato (Solanum tuberosum subsp. andigena). The gene, in which ADG2 represents the predicted nucleotide-binding domain (NBS), was cloned and characterized. The coding region of the gene (designated as Y-1) is 6,187 bp long and structurally similar to gene N that confers hypersensitive resistance to Tobacco mosaic virus in Nicotiana spp. Both belong to the TIR-NBS-LRR class of genes and show 57% identity at the amino acid sequence level. The introns of Y-1 were spliced as predicted from the sequence. Y-1 cosegregated with Ry adg, a gene for extreme resistance to Potato virus Y (PVY) on chromosome XI, as tested in a potato-mapping population and with independent potato cultivars. Leaves of the transgenic potato plants expressing Y-1 under the control of Cauliflower mosaic virus 35S promoter developed necrotic lesions upon infection with PVY, but no significant resistance was observed, and plants were systemically infected with PVY.

© 2002 The American Phytopathological Society