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Induction of Trehalase in Arabidopsis Plants Infected With the Trehalose-Producing Pathogen Plasmodiophora brassicae

July 2002 , Volume 15 , Number  7
Pages  693 - 700

David Brodmann , 1 Astrid Schuller , 2 Jutta Ludwig-Müller , 2 Roger A. Aeschbacher , 1 Andres Wiemken , 1 Thomas Boller , 1 and Astrid Wingler 1

1Botanisches Institut, Universität Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland; 2Institut für Botanik, Technische Universität Dresden, D-01062 Dresden, Germany

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Accepted 16 March 2002.

Various microorganisms produce the disaccharide trehalose during their symbiotic and pathogenic interactions with plants. Trehalose has strong effects on plant metabolism and growth; therefore, we became interested to study its possible role in the interaction of Arabidopsis thaliana with Plasmodiophora brassicae, the causal agent of clubroot disease. We found that trehalose accumulated strongly in the infected organs (i.e., the roots and hypocotyls) and, to a lesser extent, in the leaves and stems of infected plants. This accumulation pattern of trehalose correlated with the expression of a putative trehalose-6-phosphate synthase (EC gene from P. brassicae, PbTPS1. Clubroot formation also resulted in an induction of the Arabidopsis trehalase gene, ATTRE1, and in a concomitant increase in trehalase (EC activity in the roots and hypocotyls, but not in the leaves and stems of infected plants. Thus, induction of ATTRE1 expression was probably responsible for the increased trehalase activity. Trehalase activity increased before trehalose accumulated; therefore, it is unlikely that trehalase was induced by its substrate. The induction of trehalase may be part of the plant's defense response and may prevent excess accumulation of trehalose in the plant cells, where it could interfere with the regulation of carbon metabolism.

Additional keywords: ADP-glucose pyrophosphorylase , auxin , cytokinin .

© 2002 The American Phytopathological Society