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Patterns of Pectin Methylesterase Transcripts in Developing Stem Nodules of Sesbania rostrata

February 2002 , Volume 15 , Number  2
Pages  164 - 168

Sam Lievens , Sofie Goormachtig , Sylvia Herman , and Marcelle Holsters

Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

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Accepted 14 October 2001.

Differential display was applied to the early stages of the interaction between the tropical legume Sesbania rostrata and its microsymbiont Azorhizobium caulinodans ORS571. An upregulated clone that is similar to pectin methylesterase-encoding genes was isolated (Srpme1). The full-length sequence of Srpme1 was used to localize PME transcripts in situ during S. rostrata stem-nodule development. Several expression patterns were distinguished, hinting at general roles in vascular tissue development and cell division or expansion and at symbiosis-specific functions, such as uninfected cell differentiation.

Additional keywords: in situ hybridization , lateral root primordia , nodulation .

© 2002 The American Phytopathological Society