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Virus-Induced Gene Silencing in Transgenic Plants Expressing the Minor Capsid Protein of Beet western yellows virus

August 2002 , Volume 15 , Number  8
Pages  799 - 807

V. Brault , S. Pfeffer , M. Erdinger , J. Mutterer , and V. Ziegler-Graff

UR-BIVV, INRA, 28 rue de Herrlisheim 68021 Colmar, Cedex, France; IBMP, CNRS, 12 rue du Général Zimmer 67084 Strasbourg, Cedex, France

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Accepted 19 April 2002.

Transgenic Nicotiana benthamiana expressing the minor coat protein P74 of the phloem-limited Beet western yellows virus (BWYV) exhibited an unusual spatial pattern of post-transcriptional gene silencing (PTGS) when infected with BWYV or related viruses. Following infection, transgenic P74 and its mRNA accumulated to only low levels, 21 to 23 nucleotide RNAs homologous to the transgene appeared, and the transgene DNA underwent methylation. The infecting viral RNA, however, was not subject to significant silencing but multiplied readily and produced P74 in the phloem tissues, although the P74 encoded by the transgene disappeared from the phloem as well as the nonvascular tissues.

Additional keywords: polerovirus , virus-induced gene silencing .

The American Phytopathological Society, 2002