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Expressed Sequence Tags from Roots and Nodule Primordia of Lotus japonicus Infected with Mesorhizobium loti

April 2002 , Volume 15 , Number  4
Pages  376 - 379

Carsten Poulsen and Lone Pødenphant

Department of Molecular and Structural Biology, Laboratory of Gene Expression, University of Aarhus, Gustav Wieds Vej 10, DK Aarhus C, Denmark

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Accepted 3 January 2002.

Messenger RNA from young Lotus japonicus roots carrying root nodule primordia appearing after inoculation with Mesorhizobium loti bacteria were used to construct a cDNA expression library. Single-pass sequencing employing colony-polymerase chain reaction (PCR) and analysis of PCR products established a total of 2,397 new expressed sequence tags (ESTs). We have putatively identified 1,236 known and 484 hypothetical proteins coded by the corresponding mRNAs. The remaining cDNAs are unknown (316) or redundant overlapping cDNAs (361). We hope that this batch of ESTs will assist in the recognition of plant genes involved during development of nitrogen-fixing root nodules.

Additional keywords: legumes , root nodule development.

© 2002 The American Phytopathological Society