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Structural and Expression Analysis of Uricase mRNA from Lotus japonicus

October 2000 , Volume 13 , Number  10
Pages  1,156 - 1,160

Ken-ichi Takane , 1 Shigeyuki Tajima , 2 and Hiroshi Kouchi 1

1Department of Plant Physiology, National Institute of Agrobiological Resources, Tsukuba, Ibaraki, 305-8602 Japan; 2Department of Life Science, Kagawa University, Miki-cho, Kida-gun, Kagawa, 761-0795 Japan

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Accepted 23 June 2000.

Uricase (nodulin-35) cDNA, LjUr, was isolated from nodules of a model legume, Lotus japonicus. LjUr expression was most abundant in nodules, although it was detected in nonsymbiotic tissues as well, particularly in roots. Expression in nodules was detected in uninfected cells, nodule parenchyma, and, more intensely, in vascular bundles. Phylogenetic analysis of uricase sequences from various legumes indicated that uricases of amide- and ureide-transporting legumes form two distinct clades. LjUr is in the cluster of amide-transport legumes even though L. japonicus bears determinate nodules.

© 2000 The American Phytopathological Society