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Both Induction and Morphogenesis of Cyst Nematode Feeding Cells Are Mediated by Auxin

October 2000 , Volume 13 , Number  10
Pages  1,121 - 1,129

Aska Goverse , 1 Hein Overmars , 1 Jan Engelbertink , 2 Arjen Schots , 2 Jaap Bakker , 1 and Johannes Helder 1

1The Graduate School for Experimental Plant Sciences, Laboratory of Nematology, Wageningen University, Binnenhaven 10, 6709 PD, Wageningen, The Netherlands; 2The Graduate School for Experimental Plant Sciences, Laboratory for Monoclonal Antibodies, Wageningen University, Binnenhaven 10, 6709 PD, Wageningen, The Netherlands


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Accepted 7 June 2000

Various lines of evidence show that local changes in the auxin concentration are involved in the initiation and directional expansion of syncytia induced by cyst nematodes. Analysis of nematode infections on auxin-insensitive tomato and Arabidopsis mutants revealed various phenotypes ranging from complete inhibition of syncytium development to a decrease in hypertrophy and lateral root formation at the infection site. Specific activation of an auxin-responsive promoter confirmed the role of auxin and pointed at a local accumulation of auxin in developing syncytia. Disturbance of auxin gradients by inhibiting polar auxin transport with N-(1-naphthyl)phtalamic acid (NPA) resulted in abnormal feeding cells, which were characterized by extreme galling, massive disordered cell divisions in the cortex, and absence of radial expansion of the syncytium initial toward the vascular bundle. The role of auxin gradients in guiding feeding cell morphogenesis and the cross-talk between auxin and ethylene resulting in a local activation of cell wall degrading enzymes are discussed.



© 2000 The American Phytopathological Society