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Transposon Insertion in the ftsK Gene Impairs In Planta Growth and Lesion-Forming Abilities in Pseudomonas syringae pv. syringae B728a

November 2000 , Volume 13 , Number  11
Pages  1,263 - 1,265

Thomas G. Kinscherf , 1 Susan S. Hirano , 1 and David K. Willis 2

1Department of Plant Pathology, University of Wisconsin-Madison, 1630 Linden Drive, Madison 53706, U.S.A.; 2U.S. Department of Agriculture, Agricultural Research Service, University of Wisconsin-Madison, 1630 Linden Drive, Madison 53706, U.S.A.


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Accepted 20 July 2000.

A Tn5 insertion in the ftsK gene of Pseudomonas syringae pv. syringae B728a impaired brown spot lesion formation on Phaseolus vulgaris, the ability to grow within bean leaves, and swarming ability on semisolid agar. Plasmids containing the ftsK gene were sufficient to complement the original Tn5 mutant for lesion formation and swarming and partially restored in planta growth.


Additional keywords: pathogenicity, plant pathogen, virulence.

The American Phytopathological Society, 2000