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Decreased Inositol 1,4,5-Trisphosphate Content in Pathogen-Challenged Soybean Cells

May 2000 , Volume 13 , Number  5
Pages  563 - 567

Toshiro Shigaki and Madan K. Bhattacharyya

Plant Biology Division, The Samuel Roberts Noble Foundation, P.O. Box 2180, Ardmore, Oklahoma 73402, U.S.A.

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Accepted 12 January 2000.

Phosphoinositide-specific phospholipase C (PI-PLC) has been shown to be transiently activated when plant cells were treated with elicitors. We thus investigated the activity of PI-PLC when soybean cells were infected with the bacterial pathogen Pseudomonas syringae pv. glycinea, by measuring cellular cytosolic inositol 1,4,5-trisphosphate (IP3) levels. We observed that IP3 content decreased in both compatible and incompatible interactions. In vitro phosphatase activities were similar in both water control and infected cells with slightly lower IP3 degradation observed for infected cells, indicating that the reduced IP3 content in infected cells most likely results from reduced PI-PLC activity. We hypothesize that reduced IP3 content following infection may lead to suppression of various housekeeping activities of the cells, thus diverting the cellular resources either to the synthesis of defense-related compounds against pathogens, and/or to the growth of pathogens.

Additional keywords: oxidative burst, signal transduction.

© 2000 The American Phytopathological Society