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Host Suppressors in Arabidopsis thaliana of Mutations in the Movement Protein Gene of Cauliflower mosaic virus

May 2000 , Volume 13 , Number  5
Pages  512 - 519

Anton S. Callaway Zhong Huang , and Stephen H. Howell

Boyce Thompson Institute, Tower Road, Cornell University, Ithaca, NY 14853, U.S.A.

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Accepted 3 February 2000.

A novel genetic screen was used to identify host factors in Arabidopsis thaliana that suppress mutations in the Cauliflower mosaic virus (CaMV) movement protein gene (gene I). A series of small mutations was made in gene I and the mutations were tested for their suitability in a suppressor screen. The first round of screening yielded only revertants or second-site mutations in gene I. A derivative of one of the second-site mutant viruses (N7) that was delayed in symptom production was used in a second round of screening for suppressor plants that accelerated symptom production. Two candidate suppressor plants were found that accelerated by 1 to 4 days the first appearance of symptoms caused by the mutant viruses. One of the suppressors (5-2), called asc1 (acceleration of symptoms by CaMV N7), was mapped to chromosome 1. Two additional loci that differentially affect N7 virus susceptibility in the parental Columbia and Ler ecotypes were mapped to chromosomes 3 and 4 by quantitative trait locus (QTL) analysis.

Additional keywords: ecotypic variation, host-virus interactions, virus movement.

© 2000 The American Phytopathological Society