1Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater 74078, U.S.A.; 2Botany Department, Oklahoma State University, Stillwater 74075, U.S.A.; 3Sainsbury Laboratory, Norwich Research Park, Colney NR4 7UH, U.K.
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Accepted 3 March 2000.
The 25K, 12K, and 8K proteins and coat protein (CP) of Potato virus X (PVX) are required for virus cell-to-cell movement. In this study, experiments were conducted to determine whether the PVX 25K protein moves cell to cell and to explore potential interactions between the PVX 25K, 12K, and 8K proteins and CP. The PVX 25K gene was fused to the green fluorescent protein (GFP) gene and inserted into plasmids adjacent to the cauliflower mosaic virus 35S promoter. These plasmids were introduced by biolistic bombardment to transgenic tobacco expressing the PVX 12K, 8K, and CP genes. The GFP:25K fused proteins moved cell to cell on nontransgenic tobacco and tobacco expressing either the 12K or 8K proteins. However, the GFP:25K proteins did not move on transgenic tobacco expressing the combined 12K/8K genes or the CP gene. Thus, movement of the PVX 25K protein through plas-modesmata may be regulated by interactions with other PVX proteins.
triple gene block proteins,
viral movement protein.
© 2000 The American Phytopathological Society