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Localized Expression of Cathepsin B-like Sequences from Root Nodules of Pea (Pisum sativum)

July 2000 , Volume 13 , Number  7
Pages  778 - 780

Jason L. Vincent , Preeti Dahiya , and Nicholas J. Brewin

Department of Genetics, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, U.K.

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Accepted 5 April 2000.

Cathepsin B is an ancient family of eukaryotic cysteine proteases. We describe PsCat1, a plant cathepsin B-like transcript, identified as an expressed sequence in Rhizobium-induced, nitrogen-fixing root nodules of pea. In situ hybridization studies in root nodules showed strong, extremely localized expression of PsCat1 in individual cells associated with the central infected tissue. Restriction fragment polymorphism mapping of the PsCat1 locus in pea shows no correlation with existing mutant lines defective in symbiosis.

Additional keywords: hypersensitive response, nitrogen fixation, nodulins.

© 2000 The American Phytopathological Society