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Nodule Invasion and Symbiosome Differentiation During Rhizobium etli-Phaseolus vulgaris Symbiosis

July 2000 , Volume 13 , Number  7
Pages  733 - 741

Michele Cermola , Elena Fedorova , Rosarita Taté , Anna Riccio , Renee Favre , and Eduardo J. Patriarca

International Institute of Genetics and Biophysics, CNR, Via Marconi 10, 80125 Naples, Italy

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Accepted 5 April 2000.

By means of a detailed ultrastructural analysis of nodules induced by Rhizobium etli on the roots of Phaseolus vulgaris, we observe that the development of host-invaded cells is not synchronous. An accumulation of mitochondria was found in freshly invaded host cells, containing only a few symbiosomes (SBs) that are released from highly branched intracellular ramification of the infection threads. Moreover, besides the fusion between the SB membrane with host secretory vesicles, we observe also a great number of fusions between the outer leaflets of adjoining SB membranes, thus resulting in structures that resemble the tight junction network (zona occludens with a five-layered structure) of epithelian cells. This process was found to be induced strongly and earlier both in the invaded host cells of ineffective nodules (elicited by Fix-mutant strains of R. etli) and in the older (senescence) invaded cells of effective nodules, whereas bacteroid division is seldom if ever observed. Our observations strongly suggest that multiple-occupancy SBs also arise by fusion of single-occupancy SBs and the physiological consequence of this process is discussed.

Additional keywords: nifA, nitrogenase activity.

© 2000 The American Phytopathological Society