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Nodule-Expressed Cyp15a Cysteine Protease Genes Map to Syntenic Genome Regions in Pisum and Medicago spp.

July 2000 , Volume 13 , Number  7
Pages  715 - 723

Jason L. Vincent , 1 Maggie R. Knox , 1 T. H. Noel Ellis , 1 Péter Kaló , 2 György B. Kiss , 2 and Nicholas J. Brewin 1

1Department of Genetics, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, U.K.; 2Institute of Genetics, P.O. Box 521, Szeged H-6701, Hungary

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Accepted 5 April 2000.

PsCyp15a is a gene that encodes a vacuolar cysteine protease expressed in wilt-induced shoots of Pisum sativum (pea) and in root nodules. To further the understanding of nodular PsCyp15a expression, a region 5′ to the coding sequence of the gene was cloned. Varying lengths of 5′ untranslated sequence were fused with the uidA coding region and introduced from Agrobacterium rhizogenes into “hairy roots” of Vicia hirsuta. In this transgenic root nodulation assay, a promoter sequence of 900 bp was sufficient to give an expression pattern indistinguishable from that obtained in pea nodules by in situ hybridization. An orthologue of PsCyp15a was cloned from nodule mRNA of Medicago sativa and a corresponding gene identified in M. truncatula was also shown to express strongly in nodules. With molecular mapping techniques, it was demonstrated that these genes map to a syntenic genome location in pea and Medicago spp., but the map positions of the Cyp15a genes cannot be correlated with existing nodulation mutants.

Additional keyword: transformation.

© 2000 The American Phytopathological Society