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Small, Stable Shuttle Vectors Based on the Minimal pVS1 Replicon for Use in Gram-Negative, Plant-Associated Bacteria

February 2000 , Volume 13 , Number  2
Pages  232 - 237

Stephan Heeb , 1 Yoshifumi Itoh , 2 Takayuki Nishijyo , 2 Ursula Schnider , 1 Christoph Keel , 1 Julie Wade , 3 Ultan Walsh , 3 Fergal O'Gara , 3 and Dieter Haas , 3

1Laboratoire de Biologie Microbienne, Université de Lausanne, CH-1015 Lausanne, Switzerland; 2Division of Applied Microbiology, National Food Research Institute, Tsukuba, Ibaraki 305-8642, Japan; 3Microbiology Department, National University of Ireland, Cork, Ireland

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Accepted 15 October 1999.

The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month.

Additional keywords: biocontrol, industrial biotransformation processes, mobilization, partitioning, rhizosphere.

© 2000 The American Phytopathological Society