February
2000
, Volume
13
, Number
2
Pages
228
-
231
Authors
James B.
Stevens
,
1
Nicola G.
de Luca
,
1
John E.
Beringer
,
2
John P.
Ringer
,
1
Kay H.
Yeoman
,
1
and
Andrew W. B.
Johnston
1
Affiliations
1School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.; 2School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, U.K.
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RelatedArticle
Accepted 29 October 1999.
Abstract
We isolated a mutant of R. leguminosarum initially on the basis of reduced production of the siderophore vicibactin on chrome azurol sulfonate (CAS)/agar indicator plates. The mutation was in the purMN operon and the mutant was shown to be an adenine auxotroph and defective for nodulation of peas. The siderophore defect appears to be trivial, being due to diminished growth of the auxotroph on agar-based minimal medium, which contains unknown contaminant(s) that allow it grow poorly. Transcriptional fusions showed that purMN was transcribed at relatively high levels in media containing purines. Expression was enhanced, approximately twofold, if purines were omitted.
JnArticleKeywords
Additional keywords:
N2 fixation,
purine auxotrophy.
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ArticleCopyright
© 2000 The American Phytopathological Society
