February
2000
, Volume
13
, Number
2
Pages
210
-
216
Authors
Susumu
Hiraga
,
1
,
4
Hiroyuki
Ito
,
1
Hiromoto
Yamakawa
,
2
,
4
Norihiro
Ohtsubo
,
4
Shigemi
Seo
,
4
Ichiro
Mitsuhara
,
3
,
4
Hirokazu
Matsui
,
1
Mamoru
Honma
,
1
and
Yuko
Ohashi
3
,
4
Affiliations
1Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Hokkaido, 060-8589 Japan; 2Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8572 Japan; 3Department of Molecular Genetics, National Institute of Agrobiological Resources, Tsukuba, Ibaraki, 305-8602 Japan; 4Core Research for Evolutional Science and Technology (CREST), Chiyoda-ku, Tokyo 101-0062, Japan
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RelatedArticle
Accepted 18 October 1999.
Abstract
In Tobacco mosaic virus (TMV)-infected tobacco plants carrying the N resistance gene, a hypersensitive reaction or response (HR) occurs to enclose the virus in the infected tissue. Although a contribution of peroxidases to the resistance has been proposed, no evidence has been presented that tobacco peroxidase genes respond to HR. Here, we describe the HR-induced expression of a tobacco peroxidase gene (tpoxC1) whose induction kinetics were slightly different from those of acidic and basic tobacco pathogenesis-related (PR) protein genes. Interestingly, tpoxC1 was insensitive to the inducers of PR genes such as salicylic acid, methyl jasmonate, and ethephon. Spermine activated tpoxC1 gene expression at a low level and both acidic and basic PR gene expression at a considerably higher level. These results indicate that the induced expression of tpoxC1 is regulated differently from that of classical tobacco PR genes in the N gene-mediated self-defense system in tobacco plants.
JnArticleKeywords
Additional keywords:
wounding.
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© 2000 The American Phytopathological Society