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Fungal Development and Induction of Defense Response Genes During Early Infection of Wheat Spikes by Fusarium graminearum

February 2000 , Volume 13 , Number  2
Pages  159 - 169

Clara Pritsch , 1 Gary J. Muehlbauer , 1 William R. Bushnell , 2 , 3 David A. Somers , 1 and Carroll P. Vance 1 , 4

1Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul 55108, U.S.A.; 2Department of Plant Pathology, University of Minnesota, St. Paul 55108, U.S.A.; 3Cereal Disease Laboratory, Agricultural Research Service, U.S. Department of Agriculture, St. Paul 55108, U.S.A.; 4Plant Science Research Unit, Agricultural Research Service, U.S. Department of Agriculture, University of Minnesota, St. Paul 55108, U.S.A.

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Accepted 8 October 1999.

Fusarium head blight (FHB) of wheat is a crippling disease that causes severe economic losses in many of the wheat-growing regions of the world. Temporal patterns of fungus development and transcript accumulation of defense response genes were studied in Fusarium graminearum-inoculated wheat spikes within the first 48 to 76 h after inoculation (hai). Microscopy of inoculated glumes revealed that the fungus appeared to penetrate through stomata, exhibited subcuticular growth along stomatal rows, colonized glume parenchyma cells, and sporulated within 48 to 76 hai. No major differences in the timing of these events were found between Sumai 3 (resistant) and Wheaton (susceptible) genotypes. In complementary experiments, RNA was extracted from spikes at several time intervals up to 48 hai and temporal expression patterns were determined for defense response genes encoding peroxidase, PR-1, PR-2 (β-1,3-glucanase), PR-3 (chitinase), PR-4, and PR-5 (thaumatin-like protein). In both genotypes, transcripts for the six defense response genes accumulated as early as 6 to 12 hai during F. graminearum infection and peaked at 36 to 48 hai. Greater and earlier PR-4 and PR-5 transcript accumulation was observed in Sumai 3, compared with Wheaton. Our results show that the timing of defense response gene induction is correlated with F. graminearum infection.

The American Phytopathological Society, 2000