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cDNA-AFLP Display for the Isolation of Peronospora parasitica Genes Expressed During Infection in Arabidopsis thaliana

August 2000 , Volume 13 , Number  8
Pages  895 - 898

Erik A. van der Biezen , Hedyani Juwana , Jane E. Parker , and Jonathan D. G. Jones

The Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, U.K.

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Accepted 1 May 2000.

To identify genes from the obligatory biotrophic oomycete Peronospora parasitica that are expressed during infection in Arabidopsis thaliana we employed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fragments from infected and non-infected leaves were separated in parallel by gel electrophoresis and displayed by autoradiography. Most differential gene fragments were derived from P. parasitica.

Additional keywords: differential display.

© 2000 The American Phytopathological Society