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A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum

April 2000 , Volume 13 , Number  4
Pages  475 - 479

Cristina Pacios Bras , 1 Meritxell Alberich Jordá , 1 André H. M. Wijfjes , 1 Marga Harteveld , 1 Nico Stuurman , 1 Jane E. Thomas-Oates , 2 and Herman P. Spaink 1

1Leiden University, Institute of Molecular Plant Sciences, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands; 2Michael Barber Centre for Mass Spectrometry, UMIST, PO Box 88, Manchester, M60 1QD UK

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Accepted 17 December 1999.

Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizo-bium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-α-(2-aminoethoxyvinyl) glycine (AVG).

Additional keywords: acetyl fucose, mass spectrometry, Nod factors.

© 2000 The American Phytopathological Society