Jonathan D. G.
1Sainsbury Laboratory, John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, U.K.; 2Department of Biology, 108 Coker Hall CB 3280, University of North Carolina, Chapel Hill 27599-3280, U.S.A.; 3Monsanto PLC, Cereal Technology Group, Maris Lane, Trumpington, Cambridge, CB2 2LQ, U.K.
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Accepted 2 December 1999.
We have previously shown that tomato Cf-9 induces an Avr9-dependent hypersensitive response (HR) in Nicotiana tabacum and potato. We show here that Cf-4 also induces an Avr4-dependent HR in two tobacco species (N. tabacum and N. benthamiana). The HR induced by Cf-4 and Cf-9 was compared in stable tobacco transgenics by a seedling lethal assay and resistance to recombinant Potato virus X expressing Avr4 or Avr9. We also compared HR induction with Agrobacterium-mediated transient expression. The Cf-4/Avr4 combination induced a more rapid HR than Cf-9/Avr9. Sensitive assays for Cf-9 and Cf-4 function should prove useful for structure/function analyses of these resistance proteins in tobacco.
© 2000 The American Phytopathological Society