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POSTERS: Proteomics / metabolomics / genomics

Secretome annotation of the citrus stem end rot fungal pathogen Lasiodiplodia theobromae
Egem Ozbudak - University of Florida. Guohong Liu- University of Florida, Liliana Cano- University of Florida, Prashant Hosmani- Boyce Thompson Institute, Guohong Liu- Fujian Academy of Agricultural Sciences, Mark Ritenour- University of Florida, Bo Liu- Fujian Academy of Agricultural Science

Fungal plant pathogens secrete an array of effector proteins in order to achieve colonization of the host plant. Lasiodiplodia theobromae is a fungal pathogen that causes soft rot of fruits shortly before or after harvest, and the disease in citrus is known as Diplodia stem end rot (SER). Our aim is to investigate the repertoire of secreted proteins (secretome) from Lasiodiplodia theobromae Lt_CITRA15 isolated from grapefruit. In this study, we sequenced and assembled the genome for the strain Lt_CITRA15 using PacBio which resulted in 475 contigs with a N50 of 178kb and with the sum of contigs equivalent to 43.3Mb. We have identified using Signalp software a set of 1,203 secreted proteins out of 10,456 predicted proteins in the genome. We will perform comparative analyses using blast with secreted proteins from L. theobromoae and the secretomes from Diplodia corticola and from the grapevine pathogen Diplodia seriata. Our long term goal is to characterize unique families of secreted proteins from L. theobromae and those shared with related species. These studies will contribute to the annotation efforts on pathogen effector proteins for this genus.