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POSTERS: Pathogen detection, quantification and diagnosis

Epidemiological studies of a complex viral infection in red ginger in Hawai?i
Adriana Larrea-Sarmiento - University of Hawaii at Manoa. Xupeng Wang- University of Hawaii at Manoa, John Hu- University of Hawaii at Manoa, James Green- University of Hawaii at Manoa, Michael Melzer- University of Hawaii at Manoa, Wayne Borth- University of Hawaii At Manoa, Rebecca Barone- University of Hawaii

Red ginger (Alpinia purpurata) is among the most popular landscape plants in Hawai?i and can be subject to serious diseases. Members of the potyvirus and badnavirus genera have been previously reported in red ginger in Hawai?i displaying mosaic, leaf streaking and severe dieback symptoms. Symptomatic plants can be infected by single and/or mixed infectious of canna yellow mottle virus (CaYMV) and banana bract mosaic virus (BBrMV), but only mosaic symptoms caused by BBrMV are easy to identify by sight. Also, in some symptomatic plants, the range of symptoms could not be attributed to either virus, whether alone or in a mixed infection, suggesting the presence of additional pathogens. The aims of this project are to characterize viral populations in red ginger, to associate virus presence with expressed symptoms, and to provide insight into the epidemiology of these diseases. Samples displaying the dieback and viral-like symptoms, as described above, were collected from several locations on the island of O?ahu. Multiple samples tested positive using degenerate potyvirus and badnavirus detection primer sets, while testing negative for BBrMV and CaYMV, respectively. Sequencing of amplicons revealed the presence of two additional viruses infecting red ginger, banana streak virus and bean common mosaic virus. The latter virus has been found infecting additional hosts in Hawai?i. Additional work is currently being done to examine the etiology of the disease using PCR-based assays to discern the involvement of the above-mentioned viruses and to develop management strategies.