Rhizoctonia solani Kühn is a genetically complex soil borne pathogen that causes pre and post-emergence damping off to a wide range of crops. However, its life cycle and pathogenesis is not thoroughly well characterized. The primary form of the pathogen in nature is mycelia. This pathogen can survive in soil for many years through forming sclerotia, which is a resting spore produced under unfavorable condition. R. solani can be cultured in different artificial media but the development of sclerotia in the artificial media in the laboratory is not always easy to study the morphology of the pathogen. Currently, R. solani is grown on barley and other grains and used as inoculum for research purposes. One of our aims is to develop a system for producing sclerotia in vitro for large scale greenhouse and field trials for studying the natural infection process in sugar beet. In order to develop sclerotia; we cultured a number of Rhizoctonia isolates on different media, such as amended CV8 media, 50% potato dextrose agar, water agar, Metalaxyl-Benomyl-Vancomycin, and cornmeal agar media. We observed variations in the colony morphology and growth pattern of different isolates. Profuse production of sclerotia observed in the amended CV8 media compared to other media. Further study will be followed by using this sclerotia to understand pathogenesis at different growth stages for different germplasm lines and varieties of sugar beet. Gene expression study between the infected tissue and mock will improve our understanding in the soil-borne pathogen and host interaction.