POSTERS: Proteomics / metabolomics / genomics
Identification of host factors interacting with the movement protein of Ourmia melon virus in Arabidopsis
Cristina Rosa - The Pennsylvania State University. Natali Ozber- The Pennsylvania State University, Massimo Turina- Institute for Sustainable Plant Protection, National Research Council, Italy, Charles Anderson- The Pennsylvania State University, Paolo Margaria- DSMZ
Ourmia melon virus (OuMV) movement protein (MP) forms tubules across the cell wall of epidermal cells of OuMV-infected N. benthamiana, and these tubules contain virions; however, at this point, it is not clear if the OuMV MP can be strictly categorized as belonging to the tubule-forming group, or if it follows a novel mixed-movement model. OuMV MP has an unusual intracellular distribution, including endoplasmic reticulum (ER), trans-Golgi network (TGN), plasma membrane, and plasmodesmata, suggesting that it possibly interacts with numerous plant host factors. It is also still unknown how OuMV MP reaches the cell periphery to enable cell-to-cell movement of OuMV, and how OuMV MP is regulated by host proteins. In this study, a total of 55 unique candidate OuMV MP-interacting proteins in Arabidopsis (A. thaliana) were identified by a maltose-binding protein (MBP) pull-down assay coupled with liquid chromatography-mass spectrometry (LC-MS/MS) analysis. Among these candidates, 12 would be novel proteins of great interest. Identified candidate OuMV MP-interacting proteins are involved in membrane trafficking, calcium signaling, transport across the plasma membrane, protein folding and homeostasis, proteolysis, and metabolism. This study provides insight to reveal missing molecular components of OuMV movement, and targets for studying virus-host interactions. A better understanding of interactions between viral and host proteins will help develop tools to better control viral diseases.