TECHNICAL SESSION: Antiviral defense mechanisms
Deciphering early transcriptome changes during Sw-5 NLR immune receptor-mediated resistance to TSWV
Norma Ordaz - University of California, Davis. Diane Ullman- University of California, Savithramma Dinesh-Kumar- University of California, Sulley Ben-Mahmoud- University of California, Davis, Ugrappa Nagalaskshmi- University of Califiornia, Davis
Tomato spotted wilt tospovirus (TSWV) is a member of the order Bunyaviriale, family Peribunyaviridae and genus Tospovirus. Two crops, highly damaged by TSWV, fresh tomato and pepper, represent 18% of the value of all U.S. vegetables. The virus is transmitted by several thrips species, the most important being Frankliniella occidentalis, the western flower thrips. There are several resistance genes for TSWV, Sw-5 being the most commonly deployed in tomato because it mediates resistance against multiple tospovirus species. The Sw-5 gene encodes an intracellular nucleotide binding leucine rich repeat (NLR) class of immune receptor that is critical in recognition of the TSWV movement protein, NSm, resulting in a hypersensitive response and containment of virus to the infection site. Our goal is to understand early transcriptional changes that occur during Sw-5-mediated resistance. We used RNA-seq to identify early transcriptional changes during Sw-5 mediated resistance against TSWV by comparing mechanical and thrips inoculated susceptible (Sw-5-/-, Santa Clara) and resistant (Sw-5+/+, CNPH-LAM147) tomato isolines. We will discuss our results on differentially expressed genes (DEGs) in resistant plants and their potential function in Sw-5-mediated resistance.