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POSTERS: Genetics of resistance

Mapping of a clubroot resistance gene from rutabaga using bulked-segregant RNA-Seq
Abdulsalam Dakouri - Agriculture and Agri-Food Canada. Fengqun Yu- Agriculture and Agri-Food Canada, Bruce Gossen- Agriculture and Agri-Food Canada, Gary Peng- Agriculture and Agri-Food Canada, Masud Karim- Agriculture and Agri-Food Canada, Susan Boyetchko- Agriculture and Agri-Food Canada

Clubroot, caused by the obligate pathogen Plasmodiophora brassicae Woronin, is an important disease of brassica crops. Use of genetic resistance is currently the most effective strategy for clubroot management. The aim of this research was to identify and map resistance to clubroot in rutabaga cultivar ‘Wilhelmsburger’ (Brassica napus). A BC1 population was developed from a cross of ‘Wilhelmsburger’ with DH16516 (susceptible canola line). The BC1 population and parental lines were tested for resistance to pathotype 3 (Williams’ system) of P. brassicae collected from canola in Alberta, Canada. Resistance and susceptibility segregated in a 1:1 ratio in the BC1, indicating that a single major gene controlled the resistance. A new mapping method based on the percentage of polymorphic variants (PPV) from bulk-segregant analysis-RNA Seq was used to map the resistance gene. In this analysis, the resistance gene is generally located on the chromosome with the highest PPV; chromosome A03 had by far the highest PPV. KASP assays are being conducted to confirm the location as chromosome A03 and to fine map the gene.